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Optical micro-tomography “OPenT” allows the study of large toadfish Halobatrachus didactylus embryos and larvae

2016Journal article Restricted access
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dc.contributor.authorFelix, Pedro M.
dc.contributor.authorGonçalves, Ania
dc.contributor.authorVicente, Joana R.
dc.contributor.authorFonseca, Paulo Jorge
dc.contributor.authorAmorim, Maria Clara Pessoa
dc.contributor.authorCosta, José Lino
dc.contributor.authorMartins, Gabriel G.
dc.date.accessioned2016-06-28T17:44:17Z
dc.date.available2016-06-28T17:44:17Z
dc.date.issued2016
dc.description.abstractBatrachoidids, which include midshipman and toadfish are less known among embryologists, but are common in other fields. They are characteristic for their acoustic communication, and develop hearing and sound production while young juveniles. They lay large benthic eggs (>5mm) with a thick chorion and adhesive disk and slow development, which are particularly challenging for studying embryology. Here we took advantage of a classical tissue clearing technique and the OPenT open-source platform for optical tomography imaging, to image a series of embryos and larvae from 3 to 30mm in length, which allowed detailed 3D anatomical reconstructions non-destructively. We documented some of the developmental stages (early and late in development) and the anatomy of the delicate stato-acoustic organs, swimming bladder and associated sonic muscles. Compared to other techniques accessible to developmental biology labs, OPenT provided advantages in terms of image quality, cost of operation and data throughput, allowing identification and quantitative morphometrics of organs in larvae, earlier and with higher accuracy than is possible with other imaging techniques.pt_PT
dc.description.sponsorshipFundação para a Ciência e a Tecnologia (FCT)pt_PT
dc.identifier.citationMechanisms of Development, 140, 19-24. doi:10.1016/j.mod.2016.03.001pt_PT
dc.identifier.doi10.1016/j.mod.2016.03.001pt_PT
dc.identifier.issn0925-4773
dc.identifier.urihttp://hdl.handle.net/10400.12/4753
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPEst-OE/MAR/U10199/2014pt_PT
dc.relationRole of acoustic signals in mate choice and male-male assessment in a strongly-vocal fish, Halobatrachus didactylus
dc.subjectToadfishpt_PT
dc.subjectInner earpt_PT
dc.subjectSwim bladderpt_PT
dc.subjectDevelopmentpt_PT
dc.subjectOPenTpt_PT
dc.subjectOptical tomographypt_PT
dc.titleOptical micro-tomography “OPenT” allows the study of large toadfish Halobatrachus didactylus embryos and larvaept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleRole of acoustic signals in mate choice and male-male assessment in a strongly-vocal fish, Halobatrachus didactylus
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F118767%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F04292%2F2013/PT
oaire.citation.conferencePlaceAmsterdampt_PT
oaire.citation.endPage24pt_PT
oaire.citation.startPage19pt_PT
oaire.citation.titleMechanisms of Developmentpt_PT
oaire.citation.volume140pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication4f07b64d-ac56-4cf9-92e0-09a654d25e31
relation.isProjectOfPublicationb45075bd-b8ca-49a6-bfec-2030828e7160
relation.isProjectOfPublication.latestForDiscovery4f07b64d-ac56-4cf9-92e0-09a654d25e31

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