MARE - Artigos em revistas internacionais
Permanent URI for this collection
Browse
Recent Submissions
- Fine‐Scale Movement Data Reveal Primarily Surface Foraging and Nocturnal Flight Activity in the Endangered Bermuda PetrelPublication . Becciu, Paolo; Patterson, Allison; Gjerdrum, Carina; Madeiros, Jeremy; Campioni, LetiziaForaging behaviour plays a fundamental role in animal fitness and population dynamics., particularly for central‐place foragers like breeding seabirds. Among Procellariiform seabirds, petrels exhibit a wide range of foraging strategies finely tuned to the patchy and unpredictable distribution of resources. The extent and remote nature of their foraging grounds makes direct observation of foraging behaviour impractical, thereby requiring the use of remote tracking technologies. We deployed miniaturised multi‐sensor biologgers and collected fine scale movement data to investigate the at‐sea behaviours of the Bermuda petrel Pterodroma cahow, a poorly studied and highly threatened gadfly petrel, specialised on mesopelagic prey. GPS‐tracking data revealed extensive foraging trips (mean ± SD: 1207 ± 305 km), in consistent directions, over remote oceanic regions. Time‐depth‐recorders provided new insights into Bermuda petrel feeding techniques suggesting that the meso‐bathypelagic prey targeted by petrels must be available in the very upper layer of the water surface, given their very limited diving activity (maximum dive depth of 1.57 m). We identified three flight‐related and three water‐associated behaviours using supervised classification approach to classify behaviour from tri‐axial acceleromtetry. Flying behaviours reflected the expected dynamic soaring flight strategy of Procellariiformes; individuals spent more than 75% of their time in flight (dynamic soaring and flap‐gliding) with dynamic soaring flight being the most common behaviour under all conditions. The behaviour classified as ‘Intensive flight’ was infrequently observed but could indicate aerial dipping, a characteristic foraging technique of Pterodroma species. The remaining time was spent in three water behaviours: active, inactive and intensive, with the latter being less common but thought to reflect scavenging and prey seizing. Flight increased during dusk and in the night, highlighting greater flight activity during night compared to the day, while water behaviours were more common during the day. While some of our findings may require further validation to confirm their relevance to foraging behaviour, our work offers new and valuable insights to consider when assessing the ecological needs of this endangered species and its potential vulnerability to offshore anthropogenic activities.
- Marine protected areas stage of establishment and level of protection are good predictors of their conservation outcomesPublication . Horta e Costa, Barbara; Benito-Abelló, Carmela de; Pike, Elizabeth; Turnbull, John; MacCarthy, Jessica; Harasta, Nikki; Fragkopoulou, Eliza; Roessger, Julia; Sullivan-Stack, Jenna; Grorud-Colvert, Kirsten; Gill, David; Morgan, Lance; Gonçalves, Emanuel J.; Zupan, Mirta; Gonçalves, Jorge M.S.; Claudet, JoachimDespite the importance of active management and strong protection in driving marine protected areas (MPA) performance, coverage area remains the sole indicator for global targets. To assess whether conservation quality lags behind quantity, we conducted a global meta-analysis of 123 MPAs. We show that MPAs’ Levels of Protection and Stages of Establishment are reliable proxies for MPAs’ ecological outcomes; hence, they are good candidates for tracking MPA quality. Actively managed MPAs have significantly higher fish density and biomass than non-protected surrounding areas, while MPAs that are only implemented do not. The effectiveness of actively managed MPAs can be maximized if they are fully or highly protected. Lightly and minimally protected areas that are only implemented can deliver negative outcomes. Our findings highlight the important interplay between stages and levels as developed in the MPA Guide and support the need to include both in conservation targets to track not only MPA quantity but also quality.
- Acoustic fish community in a biogeographic transition zone of the Northeast AtlanticPublication . Ríos, Noelia; Pereira, Jodanne; Muñoz-Duque, Sebastian; Silva, Gonçalo; Pais, Miguel Pessanha; Fonseca, Paulo J; Vieira, Manuel; Amorim, Maria Clara P; Francis JuanesAbstract Fish sounds are a significant component of marine soundscapes. Recently, passive acoustic monitoring (PAM) arose as a promising tool for ecological monitoring, but a good characterization of fish acoustic communities is still needed. This study is the first to characterize the fish acoustic community at a biogeographic transition zone in the Northeast Atlantic. The research was conducted in a marine protected area (MPA) along the Portuguese mainland coast. Based on a literature review, we identified 29 (19.3%) sound-producing fish species present at this MPA, while 70 species (46.7%) were considered potentially soniferous. Using in situ acoustic recordings to detect potential fish sounds, we found 33 putative fish sounds that were categorized using a simple dichotomous classification. The temporal and spectral features of the 13 most prevalent sound types were characterized and compared among them and with available recordings to identify similarities. Finally, hydrophone recordings coupled with baited remote underwater video systems were tested as a method to identify sound sources. This study provides the first fish sound catalogue from the Portuguese mainland coast, laying the foundations to survey fish communities in coastal habitats with PAM.
- Nutrient input from green turtle eggs and hatchlings in a west Africa Island and its nearshore environmentPublication . Ferreira Raposo, Cheila Sofia; Patrício, Ana Rita; Catry, Paulo; Barbosa, Castro; Camará, Assana; Regalla, Aissa; Rebelo, RuiSea turtle nesting brings marine-derived nutrients into sandy beach ecosystems, potentially influencing their dynamics. We investigated nutrient input from green turtle Chelonia mydas eggs and hatchlings into coastal habitats in Guinea-Bissau, West Africa. We assessed two islands within the João Vieira-Poilão Marine National Park (‘JVPMNP’), with contrasting sea turtle nesting density: very low (João Vieira) and very high (Poilão). On each island, we analyzed stable isotopes of carbon and nitrogen in one pair of plant species (coinvine Dalbergia ecastaphyllum bush/baobab Adansonia digitata tree) and two pairs of predator species (tufted ghost crab Ocypode cursor/African rainbow crab Cardisoma armatum and sea catfish Carlarius spp./crevalle jack Caranx hippos). The first species in each pair is distributed closer to nesting sites, while the second is found further inland or offshore. Stomach content analysis was also conducted for fishes. Results supported nutrient input from turtle clutches benefiting the species living near nesting sites. Shoreline coinvines at Poilão had higher δ15N (5.1 ± 3.2‰) compared to João Vieira (≈0‰), suggesting assimilation of turtle-derived 15N. Shoreline tufted ghost crabs at Poilão had enriched δ15N (15.2 ± 0.7‰ vs. João Vieira’s 12.2 ± 1.1‰) and δ13C (− 13.4 ± 0.7‰ vs. João Vieira’s − 18.7 ± 1.4‰), likely reflecting predation upon eggs and hatchlings. Sea catfishes at Poilão frequently consumed unhatched turtle eggs washed into the sea (17% frequency of occurrence) and hatchlings (up to 27%). This study suggests that nutrient availability from turtle nests influences and supports shoreline plants and consumers at JVPMNP.
- Mitonuclear genetics reveals a novel perspective on the stock structure and fishery management of skipjack tuna (Katsuwonus pelamis) in the Atlantic OceanPublication . Queiroz-Brito, Maria Clara G.; Mendonça, Fernando F.; Robalo, Joana; Arocha, Freddy; Diaha, N’Guessan Constance; Amandé, Monin Justin; Sow, Fambaye Ngom; Angueko, Davy; Travassos, Paulo; Torres, Rodrigo A.The skipjack tuna, Katsuwonus pelamis is the worldwide most fished and economically significant tuna species. In the Atlantic Ocean, the management strategy of the International Commission for the Conservation of Atlantic Tunas (ICCAT) includes two stocks: (a) eastern (SKJ-E) and (b) western (SKJ-W) stocks. However, the genetic cohesiveness between these stocks remains poorly understood. Here, we provide this information through mitochondrial [control region (CR)] and nuclear (intron S7) data. In both markers, high genetic diversity and no contraction signals in effective population sizes were found, supporting the species conservation status as ‘Least Concern’ by the IUCN and the results of the latest ICCAT assessment of the two stocks. In general, no well-defined genetic-geographic structure was detected, which may be explained by the specie life history strategies, such as large population effective sizes and migratory behavior, with a broad geographical distribution, that reduce the isolation, inbreeding, and genetic drift effects. However, pairwise FST values within and between the Eastern and Western Atlantic suggest some weak to moderate genetic differentiation. These results involved mainly the Eastern Temperate Zone, represented herein by the Azores archipelago. It is important to highlight that higher differentiation levels were found between sites from different stocks. In addition, the CR data revealed a weak but significant genetic differentiation between the eastern and western stocks (FST = 0.0006). These results can be used to improve and support the ICCAT management strategies of skipjack tuna stocks, highlighting the need for a reevaluation and possible alignment between Eastern and Western Atlantic management actions. Similar content being viewed by others
- Metastatic gastric adenocarcinoma in a grey seal Halichoerus grypus: Clinicopathological and immunohistochemical characterizationPublication . Marques, Gonçalo Nogueira; Marti-Garcia, Bernat; Leal, Miriam Oliveira; Silva, Nuno Urbani Ramos da; Flanagan, Carla Anne; Suárez-Bonnet, Alejandro; Leal, M OliveiraA 33 yr old female grey seal. Halichoerus grypus presented with inappetence and progressive weight loss. Medical management included blood analysis, imaging, and fecal evaluation, along with multimodal supportive therapy, which periodically improved the overall medical condition. Six months after the initial presentation, the clinical condition deteriorated significantly, including severe hyporexia, hematemesis, and marked neutrophilic leukocytosis, which led to the decision to euthanise based on welfare grounds. Necropsy findings included severe thickening of the distal esophagus, cardia, and proximal gastric fundus, as well as multiple nodular to cystic structures over the stomach’s serosa, omentum, and mesentery. Histologically, a mucinous gastric adenocarcinoma was diagnosed, with metastasis to the gastric lymph nodes and prominent carcinomatosis involving the omentum, mesentery, and diaphragm. Immunohistochemically, the gastric adenocarcinoma was positive for cytokeratin AE1/AE3, weakly positive for COX-2 and E-cadherin, and negative for vimentin. The Ki-67 proliferative index was low (0.8). Although rare, this case offers further insights into the clinical presentation, histopathology, and immunohistochemical profile of gastric tumors in pinnipeds.
- Migratory Connectivity and Non‐Breeding Habitat Segregation Across Biogeographical Scales in Closely Related Seabird TaxaPublication . Morera‐Pujol, Virginia; Catry, Paulo; Magalhães, Maria; Péron, Clara; Reyes‐González, José Manuel; Granadeiro, José Pedro; Militão, Teresa; Dias, Maria P.; Oro, Daniel; Igual, José Manuel; Dell'Omo, Giacomo; Müller, Martina; Paiva, Vitor H.; Metzger, Benjamin; Neves, Verónica; Navarro, Joan; Karris, Georgios; Xirouchakis, Stavros; Cecere, Jacopo G.; Zamora‐Marín, José Manuel; Forero, Manuela G.; Afán, Isabel; Ouni, Ridha; Romdhane, Mohamed Salah; Felipe, Fernanda De; Zajková, Zuzana; Cruz‐Flores, Marta; Grémillet, David; González‐Solís, Jacob; Ramos, RaülIn highly mobile species, Migratory Connectivity (MC) has relevant consequences in population dynamics, genetic mixing, conservation and management. Additionally, in colonially breeding species, the maintenance of the breeding geographical structure during the non‐breeding period, that is, a strong MC, can promote isolation and population divergence, which ultimately can affect the process of lineage sorting. In geographically structured populations, studying the MC and differences in environmental preferences among colonies, populations, or taxa can improve our understanding of the ecological divergence among them.We investigated the MC and non‐breeding ecological niche of three seabird taxa from the genus colonies, we assess the level (from taxa to colony) at which MC and non‐breeding spatial and environmental segregation emerge. At a taxon level, we found a clear difference in the non‐breeding distributions between Cory's. and Scopoli's shearwaters, and a clear ecological divergence between Cory's and Cape Verde shearwaters. At an intermediate aggregation level, we found that birds breeding in proximity had similar non‐breeding habitat preferences, while birds breeding in very distant colonies (and therefore classified in different populations) had different non‐breeding habitat preferences. Furthermore, within each taxon, we found more structure (i.e. stronger MC) and non‐breeding divergence at an intermediate aggregation level than at the colony scale, where MC was weak. Main Conclusions These results suggest that conspecifics from nearby colonies mix in common non‐breeding areas, but not with birds from more distant colonies or different taxa. These results support the need for management and conservation strategies that take into account this structure when dealing with migratory species with high connectivity.
- Bioprospecting bioactive peptides in Halobatrachus didactylus Body Mucus: From in silico insights to essential in vitro validationPublication . Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, Maria ManuelaFish body mucus plays a protective role, especially in Halobatrachus didactylus, which inhabits intertidal zones vulnerable to anthropogenic contaminants. In silico predicted bioactive peptides were identified in its body mucus, namely, EDNSELGQETPTLR (HdKTLR), DPPNPKNL (HdKNL), PAPPPPPP (HdPPP), VYPFPGPLPN (HdVLPN), and PFPGPLPN (HdLPN). These peptides were studied in vitro for bioactivities and aggregation behavior under different ionic strengths and pH values. Size exclusion chromatography revealed significant peptide aggregation at 344 mM and 700 mM ionic strengths at pH 7.0, decreasing at pH 3.0 and pH 5.0. Although none exhibited antimicrobial properties, they inhibited Pseudomonas aeruginosa biofilm formation. Notably, HdVLPN demonstrated potential antioxidant activity (ORAC: 1.560 μmol TE/μmol of peptide; ABTS: 1.755 μmol TE/μmol of peptide) as well as HdLPN (ORAC: 0.195 μmol TE/μmol of peptide; ABTS: 0.128 μmol TE/μmol of peptide). Antioxidant activity decreased at pH 5.0 and pH 3.0. Interactions between the peptides and mucus synergistically enhanced antioxidant effects. HdVLPN and HdLPN were non-toxic to Caco-2 and HaCaT cells at 100 μg of peptide/mL. HdPPP showed potential antihypertensive and antidiabetic effects, with IC50 values of 557 μg of peptide/mL for ACE inhibition and 1700 μg of peptide/mL for α-glucosidase inhibition. This study highlights the importance of validating peptide bioactivities in vitro, considering their native environment (mucus), and bioprospecting novel bioactive molecules while promoting species conservation.
- Southernmost record of Liparis montagui (Donovan, 1804) (Perciformes, Liparidae) in European waters (central Portugal), with genetic validationPublication . Duarte-Coelho, Pedro; Miranda, Carolina; Pereira, Ana; Vasco-Rodrigues, Nuno; Almada, FredericoIn January 2022, the presence of adult Liparis montagui (Donovan, 1804) was documented at its southernmost point along the west coast of Portugal. The species was identified through both morphological and genetic barcoding analyses. This observation, part of an ongoing long-term coastal survey, suggests that L. montagui may be a transient species, influenced by complex climate and oceanic interactions along the western Iberian coast. This finding stresses the importance of long-term ecological studies and regular field surveys in understanding species distribution and the effects of climate change on marine biodiversity.
- Brucella ceti in Common Dolphins (Delphinus delphis) in Portugal—Characterization of first IsolatesPublication . Cavaco, Sandra; Grilo, Miguel; Dias, Ricardo; Nunes, Mónica; Pascoal, Pedro; Pereira, Marcelo; Fogaça, Catarina; Costa, Ana Beatriz; Pardal, Sofia; Ferreira, Ana CristinaThis study investigates Brucella ceti infection in marine mammals stranded along the Lisbon and Tagus Valley coast between 2022 and mid-2024, marking the first report of Brucella presence in Portuguese waters. Out of 59 examined marine mammals, B. ceti was isolated in three common dolphins (5.1%), a prevalence rate consistent with previous studies from other coastlines. PCR-based detection indicated a higher infection rate (23.7%), suggesting an underestimation of the prevalence of B. ceti infection in this population. Multi-locus Sequence Typing (MLST) and Multiple-Locus Variable-Number Tandem-Repeat Analysis (MLVA) revealed distinct genetic profiles and close relationships to B. ceti strains from the Atlantic, supporting the hypothesis of specific host-adapted lineages in dolphins. Virulence genes, including those for host interaction (bspE, btpB) and intracellular survival (virB7, vceA), were consistent across isolates, highlighting the pathogenic potential. Additionally, antimicrobial resistance (AMR) genes, such as mprF and efflux proteins (bepC-G), were also identified. These findings underscore the need for further research and surveillance to understand B. ceti transmission, host range, and impacts on Atlantic cetaceans, as well as to develop effective diagnostic and management strategies to mitigate infection risks in marine environments.