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Publicação
Extensive intraspecific polymorphism detected by SSCP at the nuclear C-mos gene in the endemic Iberian lizard Lacerta Schreiberi
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Orientador(es)
Resumo(s)
C-mos
is a highly conserved intronless gene that has proved useful in the analysis of
ancient phylogenetic relationships within vertebrates. We selected the Iberian endemic
Schreiber’s green lizard (Lacerta schreiberi) that persisted in allopatric refugia since the late
Pliocene to investigate the utility of the Cmos
nuclear gene for intraspecific phylogeographic
studies. Our combination of DNA sequencing with the high resolving power of
single-strand conformational polymorphism (SSCP) effectively discriminated four common
alleles showing strong population structuring (FST = 0.46). In addition, reconstruction
of allele phylogenetic relationships further improved our understanding of Cmos
spatial
patterns of variation and allowed a comparison with previously described mitochondrial
DNA data. Finally, limited sequencing of an extended Cmos
fragment in six additional
Lacerta
species showed extensive polymorphism, to our knowledge representing a rare
example of variation in a highly conserved nuclear gene.
Descrição
Palavras-chave
Intraspecific polymorphism Lacerta schreiberi Nuclear gene C-mos SSCP
Contexto Educativo
Citação
Molecular Ecology, 15, 731-738