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Implementation of a Zebrafish Health Program in a Research Facility: A 4-Year Retrospective Study

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dc.contributor.authorBorges, Ana C.
dc.contributor.authorPereira, Nuno Marques
dc.contributor.authorFranco, Maysa
dc.contributor.authorVale, Liliana
dc.contributor.authorPereira, Margarida
dc.contributor.authorCunha, Mónica V.
dc.contributor.authorAmaro, Ana
dc.contributor.authorAlbuquerque, Teresa
dc.contributor.authorRebelo, Manuel
dc.date.accessioned2017-11-03T16:22:41Z
dc.date.available2017-11-03T16:22:41Z
dc.date.issued2016
dc.description.abstractIn the past two decades, zebrafish (Danio rerio)-based research has contributed to significant scientific advances. Still, husbandry and health programs did not evolve at the same pace, as evidenced by the absence of general guidelines. Health monitoring is essential to animal welfare, to permit animal exchanges across facilities, to contribute to robust experimental results, and for data reproducibility. In this study, we report a health program implemented in a zebrafish research facility to prevent, monitor, and control pathogen, and disease dissemination. This program includes quarantine, routine health screening of sentinels, and nonroutine screenings of retired animals and sick/moribund individuals. An extensive list of clinical signs, lesions, and pathogens was monitored based on: daily observation of fish, necropsy, histology, and bacterial culture. The results indicate that the combined analysis of sentinels with the evaluation of sick/moribund animals enables a comprehensive description not only of pathogen prevalence but also of clinical and histopathologic lesions of resident animals. The establishment of a quarantine program revealed to be effective in the reduction of Pseudoloma neurophilia frequency in the main aquaria room. Finally, characterization of the colony health status based on this multiapproach program shows a low prevalence of lesions and pathogens in the facility.pt_PT
dc.description.sponsorshipInstituto Gulbenkian de Ciência (IGC); Animal House Core Facility (AHCF);pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationZebrafish, S1(13), S-115-S126pt_PT
dc.identifier.doi10.1089/zeb.2015.1230pt_PT
dc.identifier.issn1545-8547
dc.identifier.urihttp://hdl.handle.net/10400.12/5881
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMary Ann Liebertpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/pt_PT
dc.subjectAnimal Welfarept_PT
dc.subjectAnimalspt_PT
dc.subjectFish Diseasespt_PT
dc.subjectMicrosporidiapt_PT
dc.subjectMicrosporidiosispt_PT
dc.subjectRetrospective Studiespt_PT
dc.subjectZebrafishpt_PT
dc.titleImplementation of a Zebrafish Health Program in a Research Facility: A 4-Year Retrospective Studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceUnited Statespt_PT
oaire.citation.endPageS-126pt_PT
oaire.citation.issueS1pt_PT
oaire.citation.startPageS-115pt_PT
oaire.citation.titleZebrafishpt_PT
oaire.citation.volume13pt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT

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