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Aeromonas spp. Prevalence, virulence, and antimicrobial resistance in an ex situ program for tThreatened freshwater fish—A pilot study with protective measures

2022Journal article Open access
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dc.contributor.authorGrilo, Miguel
dc.contributor.authorAmaro, Guadalupe
dc.contributor.authorChambel, Lélia
dc.contributor.authorMarques, Carolina S.
dc.contributor.authorMarques, Tiago A.
dc.contributor.authorGil, Fátima
dc.contributor.authorSousa-Santos, Carla
dc.contributor.authorRobalo, Joana
dc.contributor.authorOliveira, Manuela
dc.date.accessioned2022-03-21T19:59:19Z
dc.date.available2022-03-21T19:59:19Z
dc.date.issued2022
dc.description.abstractEx situ breeding programs are important conservation tools for endangered freshwater fish. However, developing husbandry techniques that decrease the likelihood of disease, antimicrobial resistance, and virulence determinants acquisition during this process is challenging. In this pilot study, we conducted a captivity experiment with Portuguese nase (Iberochondrostoma lusitanicum), a critically endangered leuciscid species, to investigate the influence of simple protective measures (i.e., material disinfection protocols and animal handling with gloves) on the dynamics of a potential pathogenic genus, Aeromonas, as well as its virulence profiles and antimicrobial resistance signatures. Our findings show that antimicrobial resistance in Aeromonas spp. collected from I. lusitanicum significantly increased during the extent of the assay (5 weeks), with all isolates collected at the end of the study classified as multidrug-resistant. Additionally, humans handling fishes without protective measures were colonized by Aeromonas spp. The use of protective measures suggested a decreasing trend in Aeromonas spp. prevalence in I. lusitanicum, while bacterial isolates displayed significantly lower virulence index values when virulence phenotypical expression was tested at 22 °C. Despite this study representing an initial trial, which needs support from further research, protective measures tested are considered a simple tool to be applied in ex situ breeding programs for aquatic animals worldwide. Furthermore, current results raise concern regarding antimicrobial resistance amplification and zoonotic transmission of Aeromonas spp. in aquatic ex situ programs.pt_PT
dc.description.sponsorshipFundação para a Ciência e Tecnologia - FCTpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGrilo, M. L., Amaro, G., Chambel, L., Marques, C. S., Marques, T. A., Gil, F., Sousa-Santos, C., Robalo, J. I., & Oliveira, M. (2022). Aeromonas spp. Prevalence, Virulence, and Antimicrobial Resistance in an Ex Situ Program for Threatened Freshwater Fish—A Pilot Study with Protective Measures. Animals (2076-2615), 12(4), 436. https://doi.org/10.3390/ani12040436pt_PT
dc.identifier.doi10.3390/ani12040436pt_PT
dc.identifier.issn20762615
dc.identifier.urihttp://hdl.handle.net/10400.12/8620
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPI Multidisciplinary Digital Publishing Institutept_PT
dc.relationMARE/UIDB/MAR/04292/2020pt_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relationCentre of Statistics and its Applications
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEx situpt_PT
dc.subjectAeromonas spppt_PT
dc.subjectIberochondrostoma lusitanicumpt_PT
dc.subjectVirulencept_PT
dc.subjectAntimicrobial resistancept_PT
dc.titleAeromonas spp. Prevalence, virulence, and antimicrobial resistance in an ex situ program for tThreatened freshwater fish—A pilot study with protective measurespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardTitleCentre of Statistics and its Applications
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00006%2F2020/PT
oaire.citation.conferencePlaceSwitzerlandpt_PT
oaire.citation.endPage17pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleAnimalspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGrilo
person.familyNameMarcão Chambel
person.familyNameSegurado Marques
person.familyNameMarques
person.familyNameGil
person.familyNameSousa Santos
person.familyNameRobalo
person.familyNameOliveira
person.givenNameMiguel
person.givenNameLélia Mariana
person.givenNameCarolina
person.givenNameTiago A.
person.givenNameFátima
person.givenNameCarla
person.givenNameJoana
person.givenNameManuela
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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