Repository logo
 
Publication

A noninvasive eDNA tool for detecting sea lamprey larvae in river sediments: analytical validation and field testing in a low‐abundance ecosystem

2022Journal article Open access
Simple item page
dc.contributor.authorBaltazar-Soares, Miguel
dc.contributor.authorPinder, Adrian C.
dc.contributor.authorHarrison, Andrew J.
dc.contributor.authorOliver, Will
dc.contributor.authorPicken, Jessica
dc.contributor.authorBritton, J. Robert
dc.contributor.authorAndreou, Demetra
dc.date.accessioned2022-06-07T15:04:45Z
dc.date.available2022-06-07T15:04:45Z
dc.date.issued2022
dc.description.abstractAnthropogenic activities are increasingly threatening aquatic biodiversity, especially anadromous species. Monitoring and conservation measures are thus required to protect, maintain and restore imperilled populations. While many species can be surveyed using traditional capture and visual census techniques, species that use riverine habitats in a less conspicuous manner, such as sea lamprey Petromyzon marinus, can be more challenging to monitor. Sea lamprey larvae (ammocoetes) can spend several years in freshwater burrowed within soft sediments, inhibiting their detection and assessment. Here, we present a qPCR assay based on the detection of environmental DNA (eDNA) to identify the presence of ammocoetes burrowed in the sediment. We present an extensively validated method that ensured both speciesspecificity of the assay as well as the capacity to detect ammocoetes when abundances are low. Experiments on burrowing activity suggested that most of the DNA released into the sediment occurs during burrowing. Overall, we demonstrate this new molecular-based tool is an efficient and effective complement to traditional monitoring activities targeting larval stages of sea lampreys.pt_PT
dc.description.sponsorshipFundação para a Ciência e Tecnologia - FCTpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBaltazar, S. M., Pinder, A. C., Harrison, A. J., Oliver, W., Picken, J., Britton, J. R., & Andreou, D. (2022). A noninvasive eDNA tool for detecting sea lamprey larvae in river sediments: Analytical validation and field testing in a low‐abundance ecosystem. Journal of Fish Biology, 1. https://doi.org/10.1111/jfb.15056pt_PT
dc.identifier.doi10.1111/jfb.15056pt_PT
dc.identifier.issn00221112
dc.identifier.urihttp://hdl.handle.net/10400.12/8686
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWiley-Blackwell Publishing Ltdpt_PT
dc.relationMARE - Marine and Environmental Sciences Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiological conservationpt_PT
dc.subjectEnvironmental DNApt_PT
dc.subjectFreshwater ecologypt_PT
dc.subjectMonitoringpt_PT
dc.subjectSea lampreypt_PT
dc.titleA noninvasive eDNA tool for detecting sea lamprey larvae in river sediments: analytical validation and field testing in a low‐abundance ecosystempt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMARE - Marine and Environmental Sciences Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMAR%2F04292%2F2013/PT
oaire.citation.conferencePlaceUnited Kingdompt_PT
oaire.citation.endPage9pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleJournal of Fish Biologypt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameBaltazar-Soares
person.familyNameHarrison
person.familyNameAndreou
person.givenNameMiguel
person.givenNameAndrew J.
person.givenNameDemetra
person.identifier.orcid0000-0002-5919-6197
person.identifier.orcid0000-0003-2202-361X
person.identifier.orcid0000-0001-9242-4692
person.identifier.scopus-author-id55972343000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationada74091-8ed2-41d2-8962-f97af24b94c3
relation.isAuthorOfPublicationbea680d7-87ac-44d5-9750-cd68c657744b
relation.isAuthorOfPublication4c8e69c9-9654-4d53-ae2d-29cceaf4d77d
relation.isAuthorOfPublication.latestForDiscovery4c8e69c9-9654-4d53-ae2d-29cceaf4d77d
relation.isProjectOfPublicationf827860f-7fed-40b5-a4f2-24ab09a14195
relation.isProjectOfPublication.latestForDiscoveryf827860f-7fed-40b5-a4f2-24ab09a14195

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Journal of Fish Biology - 2022 - Baltazar‐Soares - A noninvasive eDNA tool for detecting sea lamprey larvae in river (1).pdf
Size:
822.54 KB
Format:
Adobe Portable Document Format
Download

Collections

MARE - Artigos em revistas internacionais