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Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)

dc.contributor.authorMarum, Liliana
dc.contributor.authorRocheta, Margarida
dc.contributor.authorMaroco, João
dc.contributor.authorOliveira, Maria Margarida
dc.contributor.authorMiguel, Célia M.
dc.date.accessioned2012-09-22T12:19:24Z
dc.date.available2012-09-22T12:19:24Z
dc.date.issued2009
dc.description.abstractSomatic embryogenesis (SE) is a propagation tool of particular interest for accelerating the deployment of new high-performance planting stock in multivarietal forestry. However, genetic conformity in in vitro propagated plants should be assessed as early as possible, especially in long-living trees such as conifers. The main objective of this work was to study such conformity based on genetic stability at simple sequence repeat (SSR) loci during somatic embryogenesis in maritime pine (Pinus pinaster Ait.). Embryogenic cell lines (ECLs) subjected to tissue proliferation during 6, 14 or 22 months, as well as emblings regenerated from several ECLs, were analyzed. Genetic variation at seven SSR loci was detected in ECLs under proliferation conditions for all time points, and in 5 out of 52 emblings recovered from somatic embryos. Three of these five emblings showed an abnormal phenotype consisting mainly of plagiotropism and loss of apical dominance. Despite the variation found in somatic embryogenesis-derived plant material, no correlation was established between genetic stability at the analyzed loci and abnormal embling phenotype, present in 64% of the emblings. The use of microsatellites in this work was efficient for monitoring mutation events during the somatic embryogenesis in P. pinaster. These molecular markers should be useful in the implementation of new breeding and deployment strategies for improved trees using SE.por
dc.identifier.citationPlant Cell Reports, 28, 673-682por
dc.identifier.issn0721-7714
dc.identifier.urihttp://hdl.handle.net/10400.12/1720
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.subjectGenetic stabilitypor
dc.subjectSomatic embryogenesispor
dc.subjectSomaclonal variationpor
dc.subjectMicrosatellitespor
dc.subjectPinus pinasterpor
dc.titleAnalysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceHeidelbergpor
oaire.citation.endPage682por
oaire.citation.startPage673por
oaire.citation.titlePlant Cell Reportspor
oaire.citation.volume28por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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