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Publication
Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)
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Advisor(s)
Abstract(s)
Somatic embryogenesis (SE) is a propagation
tool of particular interest for accelerating the deployment
of new high-performance planting stock in multivarietal
forestry. However, genetic conformity in in vitro propagated
plants should be assessed as early as possible,
especially in long-living trees such as conifers. The main
objective of this work was to study such conformity based
on genetic stability at simple sequence repeat (SSR) loci
during somatic embryogenesis in maritime pine (Pinus
pinaster Ait.). Embryogenic cell lines (ECLs) subjected to
tissue proliferation during 6, 14 or 22 months, as well as
emblings regenerated from several ECLs, were analyzed.
Genetic variation at seven SSR loci was detected in ECLs
under proliferation conditions for all time points, and in 5
out of 52 emblings recovered from somatic embryos. Three
of these five emblings showed an abnormal phenotype
consisting mainly of plagiotropism and loss of apical
dominance. Despite the variation found in somatic
embryogenesis-derived plant material, no correlation was
established between genetic stability at the analyzed loci
and abnormal embling phenotype, present in 64% of the
emblings. The use of microsatellites in this work was
efficient for monitoring mutation events during the somatic
embryogenesis in P. pinaster. These molecular markers
should be useful in the implementation of new breeding
and deployment strategies for improved trees using SE.
Description
Keywords
Genetic stability Somatic embryogenesis Somaclonal variation Microsatellites Pinus pinaster
Citation
Plant Cell Reports, 28, 673-682