Browsing by Author "Mollo, Ernesto"
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- Calling activity and calls' temporal features inform about fish reproductive condition and spawning in three cultured Sciaenidae speciesPublication . Bolgan, Marta; Crucianelli, A.; Mylonas, C.C.; Henry, S.; Falguière, J.C.; Parmentier, E.; Mollo, ErnestoAlthough many fish species are vocal, the use of fish sounds for aquaculture management and wild population protection has not received much attention. In this study, sound production of three members of the Sciaenidae family was monitored before and during spawning in aquaculture facilities. The species examined include the meagre Argyrosomus regius, the shi dum Umbrina cirrosa and red drum Sciaenops ocellatus. Red drum reproduces spontaneously in captivity, whereas shi drum and meagre often require hormonal stimulation using gonadotropin releasing hormone agonist (GnRHa) for the induction of oocyte maturation, ovulation and spawning. In all three species, a clear increase in calling activity was detected during spawning nights, when longer sounds with a higher number of faster repeated pulses were emitted. Changes of call temporal features could be related to histological changes in the sonic muscles. After GnRHa treatment and during spawning, meagre sonic fibres were wider and the ratio of sarcoplasmic reticulum to myofibril cross-sectional area was higher. The correlation of calling activity with spawning events opens up the possibility of using the monitoring of calling activity and of call temporal features as tools for evaluating the reproductive state of different sciaenid species, both in the wild and captivity.
- Cryptic effects of biological invasions: Reduction of the aggressive behaviour of a native fish under the influence of an "invasive" biomoleculePublication . Magliozzi, Laura; Almada, Frederico José Oliveira de; Robalo, Joana Isabel; Mollo, Ernesto; Polese, Gianluca; Gonçalves, Emanuel João; Felline, Serena; Terlizzi, Antonio; D'Aniello, BiagioThe invasive green alga Caulerpa cylindracea has become an important component of the diet of the Mediterranean white seabream Diplodus sargus. As a consequence of this ªexotic dietº, the algal bisindolic alkaloid caulerpin accumulates in the fish tissues. Although the compound shows structural similarity to endogenous indolamines that modulate animal behaviour, the potential impact of caulerpin on fish behaviour still remains unexplored. In this report, behavioural experiments both on groups and on single fish responding towards a mirror were performed under different doses of dietary caulerpin. Differences between treated and control groups for each behaviour and for the overall aggressive pattern during the different experimental phases showed that the aggressiveness of D. sargus decreased with the administration of caulerpin. These results call the attention to a still unexplored potential ability of bioactive metabolites from marine invasive species, to alter the behaviour on native species, with putative negative effects on patterns of fish growth and population dynamics
- Effect of the algal alkaloid caulerpin on neuropeptide Y (NPY) expression in the central nervous system (CNS) of Diplodus sargusPublication . Magliozzi, Laura; Maselli, Valeria; Almada, Frederico José Oliveira de; Di Cosmo, Anna; Mollo, Ernesto; Polese, GianlucaRecent studies have suggested that Mediterranean indigenous fish species are affected by bioactive metabolites coming from marine invasive species via food web interactions. In particular, both physiological and behavioural changes in the white sea bream Diplodus sargus were related to caulerpin (CAU), a bisindolic alkaloid particularly abundant in the invasive alga Caulerpa cylindracea, on which the fish actively feed. Dietary administration of CAU decreased aggressiveness in D. sargus, suggesting an anxiolytic-like effect of CAU possibly mediated by endogenous anxiolytic agents. This hypothesis is supported here by the finding of a significant increase of NPY transcriptional expression in the brain of fish fed with CAU enriched food, shedding more light on the neural mechanisms behind the altered behaviour of D. sargus.
- H NMR spectroscopy and MVA to evaluate the effects of Caulerpin-Based diet on diplodus sargus lipid profilesPublication . Del Coco, Laura; Felline, Serena; Girelli, Chiara; Angilè, Federica; Magliozzi, Laura; Almada, Frederico José Oliveira de; D’Aniello, Biagio; Mollo, Ernesto; Terlizzi, Antonio; Fanizzi, FrancescoThe biological invasion of the green algae Caulerpa cylindracea represents a serious scientific and public issue in the Mediterranean Sea, essentially due to strong modifications both to habitat structure and native benthic communities. Although alterations in health status and changes in flesh quality of some marine species (dietary exposed to C. cylindracea) have been observed, no studies on cause-effect relationships have been carried out. Here, for the first time, through a controlled feeding experiment followed by ¹H NMR Spectroscopy and multivariate analysis (PCA, OPLS-DA), we showed that caulerpin taken with diet is directly responsible of changes observed in metabolic profile of fish flesh, including alteration of lipid metabolism, in particular with a reduction of ω3 PUFA content. The potential of caulerpin to directly modulate lipid metabolism opens up new questions about causal mechanism triggered by algal metabolite also in view of a possible exploitation in the nutraceutical/medical field.