Repository logo
 
Publication

Mito-nuclear sequencing is paramount to correctly identify sympatric hybridizing fishes

2018Journal article Restricted access
Simple item page
dc.contributor.authorSantos, Carla Sousa
dc.contributor.authorPereira, Ana Martins
dc.contributor.authorBranco, Paulo
dc.contributor.authorCosta, Gonçalo J.
dc.contributor.authorSantos, José M.
dc.contributor.authorFerreira, Maria Teresa
dc.contributor.authorLima, Cristina M.
dc.contributor.authorDoadrio, Ignacio
dc.contributor.authorRobalo, Joana Isabel
dc.date.accessioned2018-11-23T19:52:27Z
dc.date.available2018-11-23T19:52:27Z
dc.date.issued2018
dc.description.abstractBackground. Hybridization may drive speciation and erode species, especially when intrageneric sympatric species are involved. Five sympatric Luciobarbus species—Luciobarbus sclateri (Günther, 1868), Luciobarbus comizo (Steindachner, 1864), Luciobarbus microcephalus (Almaça, 1967), Luciobarbus guiraonis (Steindachner, 1866), and Luciobarbus steindachneri (Almaça, 1967)—are commonly identified in field surveys by diagnostic morphological characters. Assuming that i) in loco identification is subjective and observer-dependent, ii) there is previous evidence of interspecific hybridization, and iii) the technical reports usually do not include molecular analyses, our main goal was to assess the concordance between in loco species identification based on phenotypic characters with identifications based on morphometric indices, mtDNA only, and a combination of mito-nuclear markers. Materials and methods. Specimens of Luciobarbus from six Guadiana River sub-basins were collected and sequenced for the cytochrome b and beta-actin genes. For comparative purposes, samples of Luciobarbus from other 12 river basins were also used. Four levels of taxonomical identification were conducted based on: identification made in the field (in loco identification), cytb gene only, beta-actin gene only, and mito-nuclear combined genomes. Results. Results showed that interspecific hybridization seems to be high (around 41%) and likely favoured by non-random mating and the loss of fluvial connectivity. About 34% of the hybrids showed mito-nuclear discordance. Misidentifications were frequent when only phenotypic characters are considered, and the use of a single mitochondrial gene is not sufficient: the use of two mito-nuclear markers showed that around 82% of the in loco identifications based on the phenotype were not correct. Conclusion. Incorrect species assignment likely generated biased results in previous studies on the biology and ecology of Guadiana barbels and in the assignment of conservation status and, consequently, on the establishment of conservation management measures.pt_PT
dc.description.sponsorshipEuropean Fund for Economic and Regional Development (FEDER); Program Operational Factors of Competitiveness (COMPETE); Portuguese Foundation of Science and Technology (FCT)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationActa Ichthyologica et Piscatoria.48(2), 123-141 Doi: 10.3750/AEIP/02348pt_PT
dc.identifier.doi10.3750/AEIP/02348pt_PT
dc.identifier.issn01371592
dc.identifier.urihttp://hdl.handle.net/10400.12/6732
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherScientific Society of Szczecinpt_PT
dc.relationUI&D331/94pt_PT
dc.relationSFRH/ BPD/29774/2006pt_PT
dc.relationMARE-ISPA/BPD/001/2015pt_PT
dc.relationMARE-ISPA/BI/004/2015pt_PT
dc.relationSFRH/ BPD/94686/2013pt_PT
dc.relationMARS/BI/2/2014pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectMito-nuclear incongruencept_PT
dc.subjectIntrogressionpt_PT
dc.subjectHybridizationpt_PT
dc.subjectPhenotypic traitspt_PT
dc.subjectConservationpt_PT
dc.titleMito-nuclear sequencing is paramount to correctly identify sympatric hybridizing fishespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F04292%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00239%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAC-CLI%2F103110%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0239%2F2014/PT
oaire.citation.conferencePlacePolandpt_PT
oaire.citation.endPage141pt_PT
oaire.citation.issue48pt_PT
oaire.citation.startPage123pt_PT
oaire.citation.titleActa Ichthyologica et Piscatoriapt_PT
oaire.citation.volume2pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationb45075bd-b8ca-49a6-bfec-2030828e7160
relation.isProjectOfPublication7337c81c-2d73-4b3d-a5b8-27aba5bb2ce7
relation.isProjectOfPublication69a2f463-e3d7-4818-aa91-0d9396671c9f
relation.isProjectOfPublicationa4b9907d-be8b-445f-bacd-905ff327f5e4
relation.isProjectOfPublication.latestForDiscovery69a2f463-e3d7-4818-aa91-0d9396671c9f

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ACTA ICHTHYOLOGICA ET PISCATORIA (2018) 48 (2) 123–141.pdf
Size:
5.35 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

MARE - Artigos em revistas internacionais