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Improved in vitro rooting of Prunus dulcis Mill. cultivars

dc.contributor.authorTereso, Susana
dc.contributor.authorMiguel, Célia M.
dc.contributor.authorMascarenhas, M.
dc.contributor.authorRoque, A.
dc.contributor.authorTrindade, H.
dc.contributor.authorMaroco, João
dc.contributor.authorOliveira, Maria Margarida
dc.date.accessioned2012-09-20T19:17:55Z
dc.date.available2012-09-20T19:17:55Z
dc.date.issued2008
dc.description.abstractA highly reproducible system was developed for efficient rooting of cultivars Boa Casta (BC) and Peneda and a BC seedling-derived clone (BC VII) of almond (Prunus dulcis Mill.). Twenty-four accessions derived from the clone BC VII and subjected to various in vitro culture treatments were screened. The long induction pre-treatment (LIP, 5 d), the brief induction pre-treatment (BIP, 16 h) and the hormonal shock by short dipping in hormone solution (1 min), were tested. BIP was the only that allowed rooting of cultivars. In BC VII, it induced high rooting frequencies (47 - 100 %) when using a solution of 0.4 mM indole-3-butyric acid solidified with 2 g dm-3 gellam gum for 16-h. The response to the auxin type was variable depending on the cultivar and the root induction pre-treatment used. Root number was significantly different between the two cultivars and BC VII. Root length was significantly higher when using 0.005 mM IBA in LIP but this concentration induced apical necrosis. The improved acclimatization procedure for up to 4 weeks increased the survival to 45 %. The initiation and development of adventitious roots were proved to be asynchronouspor
dc.identifier.citationBiologia Plantarum, 52 (3), 437-444por
dc.identifier.issn0006-3134
dc.identifier.urihttp://hdl.handle.net/10400.12/1708
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.subjectAdventitious rootpor
dc.subjectIndole-3-acetic acidpor
dc.subjectIndole-3-butyric acidpor
dc.subjectMother plant agepor
dc.titleImproved in vitro rooting of Prunus dulcis Mill. cultivarspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceHeidelbergpor
oaire.citation.endPage444por
oaire.citation.startPage437por
oaire.citation.titleBiologia Plantarumpor
oaire.citation.volume52por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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