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Exploring bioactivities and peptide content of body mucus from the lusitanian toadfish Halobatrachus didactylus

2023Journal article Open access
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dc.contributor.authorCunha, Marta Fernandez
dc.contributor.authorCoscueta, Ezequiel
dc.contributor.authorEmilia Brassesco, M.
dc.contributor.authorMarques, Rita
dc.contributor.authorNeto, José
dc.contributor.authorAlmada, Frederico José Oliveira de
dc.contributor.authorGoncalves, David
dc.contributor.authorPintado, Maria Manuela
dc.date.accessioned2024-02-27T19:18:43Z
dc.date.available2024-02-27T19:18:43Z
dc.date.issued2023
dc.description.abstractIdentifying bioactive molecules from marine organisms is still vastly understudied. Fish remain an untapped source of bioactive molecules, even when considering species whose toxicity to other fish species has been noticed before. We assessed potential applications of crude body mucus of the Lusitanian toadfish (Halobratachus didactylus) and characterized its peptide fraction composition. Mucus samples from three individuals (two wild and one captive) revealed potential antioxidant, antihypertensive, and antimicrobial activities. For antioxidant activity, the best results of 2371 ± 97 µmol Trolox Equivalent/g protein for ORAC and 154 ± 6 µmol Trolox Equivalent/g protein for ABTS were obtained. For antihypertensive activity, the relevant inhibitory activity of ACE resulted in IC50 of 60 ± 7 µg protein/mL. Antimicrobial activity was also identified against the pathogenic bacteria Escherichia coli and Listeria monocytogenes. The peptide profile of the crude body mucus was obtained through size exclusion chromatography, with a conspicuous peak at ca. 800 Da. LC-MS/MS allowed the detection of the most probable peptide sequences of this dominant peptide. This is the first study where the bioactive potential of mucus from the Lusitanian toadfish is demonstrated. Peptides with such properties can be applied in the food and pharmaceutical industries.pt_PT
dc.description.sponsorshipFundação Para a Ciência e Tecnologia - FCTpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFernandez Cunha, M., Coscueta, E. R., Brassesco, M. E., Marques, R., Neto, J., Almada, F., Gonçalves, D., & Pintado, M. (2023). Exploring Bioactivities and Peptide Content of Body Mucus from the Lusitanian Toadfish Halobatrachus didactylus. Molecules, 28(18), 6458. https://doi.org/10.3390/molecules28186458pt_PT
dc.identifier.doi10.3390/molecules28186458pt_PT
dc.identifier.issn14203049
dc.identifier.urihttp://hdl.handle.net/10400.12/9620
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationDL57/2016/CP1339/CT0003pt_PT
dc.relationUIDB/04292/2020pt_PT
dc.relationLA/P/0069/2020pt_PT
dc.relationFishVen - Fish venom diversity in Atlanto-Mediterranean scorpionfish Scorpaena. Venomics, evolution and impact on fisheries.
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectIchthyocrinotoxinspt_PT
dc.subjectAntimicrobialpt_PT
dc.subjectAntioxidantpt_PT
dc.subjectAntihypertensivept_PT
dc.subjectPeptidespt_PT
dc.titleExploring bioactivities and peptide content of body mucus from the lusitanian toadfish Halobatrachus didactyluspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleFishVen - Fish venom diversity in Atlanto-Mediterranean scorpionfish Scorpaena. Venomics, evolution and impact on fisheries.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/UI%2FBD%2F150955%2F2021/PT
oaire.citation.conferencePlaceSwitzerlandpt_PT
oaire.citation.issue18pt_PT
oaire.citation.startPage6458pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume28pt_PT
oaire.fundingStreamPOR_CENTRO
person.familyNameFernandez Cunha
person.familyNameCoscueta
person.familyNameBrassesco
person.familyNameAlmada
person.familyNameGoncalves
person.familyNamePintado
person.givenNameMarta
person.givenNameEzequiel
person.givenNameMaria Emilia
person.givenNameFrederico
person.givenNameDavid
person.givenNameMaria Manuela
person.identifierJ-7033-2016
person.identifierR-4684-2019
person.identifierF-5696-2013
person.identifier.ciencia-id6614-705D-EBA7
person.identifier.ciencia-id4819-BAD3-AF07
person.identifier.ciencia-id0215-02F9-6D3E
person.identifier.ciencia-id331D-3437-C4CD
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0001-5935-8670
person.identifier.orcid0000-0003-4496-2001
person.identifier.orcid0000-0001-9027-4643
person.identifier.orcid0000-0002-1389-8951
person.identifier.orcid0000-0001-6851-7215
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridJ-7033-2016
person.identifier.ridL-3514-2016
person.identifier.scopus-author-id56986455000
person.identifier.scopus-author-id57190497489
person.identifier.scopus-author-id7005392651
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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