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Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus

dc.contributor.authorSantos, Carla Sousa
dc.contributor.authorFonseca, Paulo Jorge
dc.contributor.authorAmorim, Maria Clara Pessoa
dc.date.accessioned2015-02-20T19:32:12Z
dc.date.available2015-02-20T19:32:12Z
dc.date.issued2015
dc.description.abstractThe Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locus varied between 2 and 4, and the observed heterozygosity ranged from 0.082 to 0.600. No significant deviation from Hardy–Weinberg equilibrium was found and there was no evidence for linkage disequilibrium. These markers will be of great value for paternity studies and population genetics of this species.por
dc.description.sponsorshipFundação para a Ciência e Tecnologia (FCT)por
dc.identifier.citationPeerJ, 3, e731por
dc.identifier.doi10.7717/peerj.731
dc.identifier.issn2167-8359
dc.identifier.urihttp://hdl.handle.net/10400.12/3419
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherPeerJpor
dc.relationPTDC/MAR/118767/2010)por
dc.subjectBatrachoididaepor
dc.subjectPaternitypor
dc.subjectPolymorphic locipor
dc.subjectMicrosatellite developmentpor
dc.subjectPyrosequencingpor
dc.titleDevelopment and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactyluspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceLondonpor
oaire.citation.endPage9por
oaire.citation.startPage1por
oaire.citation.titlePeerJpor
oaire.citation.volume3por
rcaap.rightsopenAccesspor
rcaap.typearticlepor

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