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Bioprospecting bioactive peptides in Halobatrachus didactylus Body Mucus: From in silico insights to essential in vitro validation

datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorCunha, Marta Fernandez
dc.contributor.authorCoscueta, Ezequiel R.
dc.contributor.authorBrassesco, María Emilia
dc.contributor.authorAlmada, Frederico
dc.contributor.authorGonçalves, David
dc.contributor.authorPintado, Maria Manuela
dc.date.accessioned2025-07-04T18:20:07Z
dc.date.available2025-07-04T18:20:07Z
dc.date.issued2025-02-13
dc.description.abstractFish body mucus plays a protective role, especially in Halobatrachus didactylus, which inhabits intertidal zones vulnerable to anthropogenic contaminants. In silico predicted bioactive peptides were identified in its body mucus, namely, EDNSELGQETPTLR (HdKTLR), DPPNPKNL (HdKNL), PAPPPPPP (HdPPP), VYPFPGPLPN (HdVLPN), and PFPGPLPN (HdLPN). These peptides were studied in vitro for bioactivities and aggregation behavior under different ionic strengths and pH values. Size exclusion chromatography revealed significant peptide aggregation at 344 mM and 700 mM ionic strengths at pH 7.0, decreasing at pH 3.0 and pH 5.0. Although none exhibited antimicrobial properties, they inhibited Pseudomonas aeruginosa biofilm formation. Notably, HdVLPN demonstrated potential antioxidant activity (ORAC: 1.560 μmol TE/μmol of peptide; ABTS: 1.755 μmol TE/μmol of peptide) as well as HdLPN (ORAC: 0.195 μmol TE/μmol of peptide; ABTS: 0.128 μmol TE/μmol of peptide). Antioxidant activity decreased at pH 5.0 and pH 3.0. Interactions between the peptides and mucus synergistically enhanced antioxidant effects. HdVLPN and HdLPN were non-toxic to Caco-2 and HaCaT cells at 100 μg of peptide/mL. HdPPP showed potential antihypertensive and antidiabetic effects, with IC50 values of 557 μg of peptide/mL for ACE inhibition and 1700 μg of peptide/mL for α-glucosidase inhibition. This study highlights the importance of validating peptide bioactivities in vitro, considering their native environment (mucus), and bioprospecting novel bioactive molecules while promoting species conservation.eng
dc.identifier.citationFernandez Cunha, M., Coscueta, E. R., Brassesco, M. E., Pintado, M. M., Almada, F., & Gonçalves, D. (2025). Bioprospecting bioactive peptides in Halobatrachus Didactylus Body Mucus: From in silico insights to essential in vitro validation. Marine Drugs, 23(2). https://doi.org/10.3390/md23020082
dc.identifier.doi10.3390/md23020082
dc.identifier.issn1660-3397
dc.identifier.urihttp://hdl.handle.net/10400.12/13553
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI AG
dc.relation.ispartofMarine Drugs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleBioprospecting bioactive peptides in Halobatrachus didactylus Body Mucus: From in silico insights to essential in vitro validation
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage13
oaire.citation.issue2
oaire.citation.startPage57
oaire.citation.titleMarine Drugs
oaire.citation.volume23
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAlmada
person.givenNameFrederico
person.identifier.ciencia-id0215-02F9-6D3E
person.identifier.orcid0000-0002-1389-8951
relation.isAuthorOfPublicationa19a10df-787c-46d8-8609-e6d2bed22ea3
relation.isAuthorOfPublication.latestForDiscoverya19a10df-787c-46d8-8609-e6d2bed22ea3

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