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Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions’ prevalence

2021Journal article Open access
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dc.contributor.authorGrilo, Miguel
dc.contributor.authorChambel, Lélia
dc.contributor.authorMarques, Tiago A.
dc.contributor.authorSousa, Carla
dc.contributor.authorRobalo, Joana
dc.contributor.authorOliveira, Manuela
dc.date.accessioned2021-12-23T19:09:24Z
dc.date.available2021-12-23T19:09:24Z
dc.date.issued2021
dc.description.abstractAssessments regarding health aspects of Iberian leuciscids are limited. There is currently an information gap regarding effects of infectious diseases on these populations and their role as a possible conservation threat. Moreover, differences in susceptibility to particular agents, such as Aeromonas spp., by different species/populations is not clear. To understand potential differences in Aeromonas diversity and load, as well as in the prevalence and proportion of skin lesions, in fishes exposed to similar environmental conditions, an observational study was implemented. Using a set of 12 individuals belonging to two sympatric Iberian leuciscid species (Squalius pyrenaicus and Iberochondrostoma lusitanicum), the skin lesion score in each individual was analyzed. Furthermore, a bacterial collection of Aeromonas spp. isolated from each individual was created and isolates’ load was quantified by plate counting, identified at species level using a multiplex-PCR assay and virulence profiles established using classical phenotypic methods. The similarity relationships of the isolates were evaluated using a RAPD analysis. The skin lesion score was significantly higher in S. pyrenaicus, while the Aeromonas spp. load did not differ between species. When analyzing Aeromonas species diversity between fishes, different patterns were observed. A predominance of A. hydrophila was detected in S. pyrenaicus individuals, while I. lusitanicum individuals displayed a more diverse structure. Similarly, the virulence index of isolates from S. pyrenaicus was higher, mostly due to the isolated Aeromonas species. Genomic typing clustered the isolates mainly by fish species and skin lesion score. Specific Aeromonas clusters were associated with higher virulence indexes. Current results suggest potential differences in susceptibility to Aeromonas spp. at the fish species/individual level, and constitute important knowledge for proper wildlife management through the signalization of at-risk fish populations and hierarchization of conservation measures.pt_PT
dc.description.sponsorshipFundação para a Cência e Tecnologia - FCTpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGrilo, M. L., Chambel, L., Marques, T. A., Sousa-Santos, C., Robalo, J. I., & Oliveira, M. (2021). Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions’ prevalence. PLoS ONE, 16(8), 1–16. https://doi.org/10.1371/journal.pone.0255850pt_PT
dc.identifier.doi10.1371/journal.pone.0255850pt_PT
dc.identifier.issn19326203
dc.identifier.urihttp://hdl.handle.net/10400.12/8387
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPublic Library of Sciencept_PT
dc.relationUIDB/MAR/04292/2020pt_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relationCentre of Statistics and its Applications
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleSympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions’ prevalencept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardTitleCentre of Statistics and its Applications
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00006%2F2020/PT
oaire.citation.conferencePlaceUnited Statespt_PT
oaire.citation.endPage16pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePLOS ONEpt_PT
oaire.citation.volume16pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGrilo
person.familyNameMarcão Chambel
person.familyNameMarques
person.familyNameSousa
person.familyNameRobalo
person.familyNameOliveira
person.givenNameMiguel
person.givenNameLélia Mariana
person.givenNameTiago A.
person.givenNameCarla
person.givenNameJoana
person.givenNameManuela
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person.identifier.orcid0000-0001-7364-821X
person.identifier.orcid0000-0002-1672-1473
person.identifier.orcid0000-0002-2581-1972
person.identifier.orcid0000-0001-6072-0825
person.identifier.orcid0000-0002-7470-0574
person.identifier.orcid0000-0002-3910-1525
person.identifier.ridN-9960-2014
person.identifier.scopus-author-id57195729611
person.identifier.scopus-author-id6503947335
person.identifier.scopus-author-id6603703024
person.identifier.scopus-author-id6506759340
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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