Publication . Martins, Tânia D.; Valentim, Ana M.; Pereira, Nuno Marques; Antunes, Luis M.
Anaesthesia is used daily in fish experimental procedures; however, the use of an inadequate anaesthetic
protocol can compromise not only the animal’s welfare but also the reliability of results. The use of zebrafish
(Danio rerio) in biomedical research has increased in the last decades, highlighting the importance of
appropriate anaesthetic regimens for this species. This article reviews the main anaesthetic agents and
protocols used in laboratory adult zebrafish, and some of the analgesic methods to be used in this species
that still need more research. In addition, a systematized observation of signs is proposed to evaluate adult
zebrafish welfare to reduce pain and distress.
