Browsing by Author "Azevedo, Vanessa"
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- Inconsistent reporting of life experiences : what people think and how they explain it?Publication . Azevedo, Vanessa; Martins, Carla; Carvalho, Margarida; Maia, ÂngelaAbstract: Assessment of life experiences relies mainly on cross-sectional retrospective design, despite the concerns regarding inconsistent reports. Studies suggest that some individuals change their answers when asked repeatedly, but common opinions underlying this behaviour remain unknown. Our study explored personal perceptions regarding inconsistent reporting and identified associated reasons including individual, experiences-related, and design-related characteristics. Seventy-two individuals, enrolled in a longitudinal study about life experiences, answered a measure about general perceptions and involved reasons. Participants seemed to be aware that inconsistent reporting is a common behaviour, which highly impact on research. A cluster analysis revealed two clusters (i.e., variables involved versus not involved). Most disagreed that sociodemographic variables influence inconsistency, whereas memory, mood, valence, impact, mode of data collection, and interviewer features were pointed as key-variables. Our results suggest that inconsistent reporting is not straightforward and it is probably rooted in a varied and complex set of variables.
- Salt tolerant and sensitive rice varieties display differential methylome flexibility under salt stressPublication . Ferreira, Liliana J.; Azevedo, Vanessa; Maroco, João; Oliveira, Maria Margarida; Santos, Ana PaulaDNA methylation has been referred as an important player in plant genomic responses to environmental stresses but correlations between the methylome plasticity and specific traits of interest are still far from being understood. In this study, we inspected global DNA methylation levels in salt tolerant and sensitive rice varieties upon salt stress imposition. Global DNA methylation was quantified using the 5-methylcytosine (5mC) antibody and an ELISAbased technique, which is an affordable and quite pioneer assay in plants, and in situ imaging of methylation sites in interphase nuclei of tissue sections. Variations of global DNA methylation levels in response to salt stress were tissue- and genotype-dependent. We show a connection between a higher ability of DNA methylation adjustment levels and salt stress tolerance. The salt-tolerant rice variety Pokkali was remarkable in its ability to quickly relax DNA methylation in response to salt stress. In spite of the same tendency for reduction of global methylation under salinity, in the salt-sensitive rice variety IR29 such reduction was not statistically supported. In ‘Pokkali’, the salt stress-induced demethylation may be linked to active demethylation due to increased expression of DNA demethylases under salt stress. In ‘IR29’, the induction of both DNA demethylases and methyltransferases may explain the lower plasticity of DNA methylation. We further show that mutations for epigenetic regulators affected specific phenotypic parameters related to salinity tolerance, such as the root length and biomass. This work emphasizes the role of differential methylome flexibility between salt tolerant and salt sensitive rice varieties as an important player in salt stress tolerance, reinforcing the need to better understand the connection between epigenetic networks and plant responses to environmental stresses.