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Spatial heterogeneity in the Mediterranean Biodiversity Hotspot affects barcoding accuracy of its freshwater fishes

2014Journal article Restricted access
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dc.contributor.authorGeiger, Matthias F.
dc.contributor.authorHerder, Fabian
dc.contributor.authorMonaghan, Michael T.
dc.contributor.authorAlmada, Vítor Carvalho
dc.contributor.authorBarbieri, Roberta
dc.contributor.authorBariche, Michel
dc.contributor.authorBerrebi, Patrick
dc.contributor.authorBohlen, Jörg
dc.contributor.authorCasal-Lopez, Miriam
dc.contributor.authorDelmastro, Giovanni B.
dc.contributor.authorDenys, Gäel P. J.
dc.contributor.authorDettai, Agnès
dc.contributor.authorDoadrio, Ignacio
dc.contributor.authorKalogianni, Elena
dc.contributor.authorKärst, Heiko
dc.contributor.authorKottelat, Maurice
dc.contributor.authorKovacic, Marcelo
dc.contributor.authorLaporte, Martin
dc.contributor.authorLorenzoni, Massimo
dc.contributor.authorMarcic, Zoran
dc.contributor.authorÖzulug, Müfit
dc.contributor.authorPerdices, Anabel
dc.contributor.authorPerea, Silvia
dc.contributor.authorPersat, Henri
dc.contributor.authorPorcellotti, Stefano
dc.contributor.authorPuzzi, Cesare
dc.contributor.authorRobalo, Joana Isabel
dc.contributor.authorSanda, Radek
dc.contributor.authorSchneider, Michael
dc.contributor.authorSlechtova, Vendula
dc.contributor.authorStumboudi, Maria
dc.contributor.authorWalter, Simon
dc.contributor.authorFreyhof, Jörg
dc.date.accessioned2014-11-22T10:35:00Z
dc.date.available2014-11-22T10:35:00Z
dc.date.issued2014
dc.description.abstractIncomplete knowledge of biodiversity remains a stumbling block for conservation planning and even occurs within globally important Biodiversity Hotspots (BH). Although technical advances have boosted the power of molecular biodiversity assessments, the link between DNA sequences and species and the analytics to discriminate entities remain crucial. Here, we present an analysis of the first DNA barcode library for the freshwater fish fauna of the Mediterranean BH (526 spp.), with virtually complete species coverage (498 spp., 98% extant species). In order to build an identification system supporting conservation, we compared species determination by taxonomists to multiple clustering analyses of DNA barcodes for 3165 specimens. The congruence of barcode clusters with morphological determination was strongly dependent on the method of cluster delineation, but was highest with the general mixed Yule-coalescent (GMYC) model-based approach (83% of all species recovered as GMYC entity). Overall, genetic morphological discontinuities suggest the existence of up to 64 previously unrecognized candidate species. We found reduced identification accuracy when using the entire DNA-barcode database, compared with analyses on databases for individual river catchments. This scale effect has important implications for barcoding assessments and suggests that fairly simple identification pipelines provide sufficient resolution in local applications. We calculated Evolutionarily Distinct and Globally Endangered scores in order to identify candidate species for conservation priority and argue that the evolutionary content of barcode data can be used to detect priority species for future IUCN assessments. We show that large-scale barcoding inventories of complex biotas are feasible and contribute directly to the evaluation of conservation priorities.por
dc.description.sponsorshipJoint Initiative for Research and Innovation (PAKT); Czech Ministry of Culture; Leibniz Associationpor
dc.identifier.citationMolecular Ecology Resources, 14(6), 1210-1221por
dc.identifier.doi10.1111/1755-0998.12257
dc.identifier.issn1755-098X
dc.identifier.urihttp://hdl.handle.net/10400.12/3213
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWiley-Blackwellpor
dc.relationDKRVO2012por
dc.relationDKRVO2013/14por
dc.relation00023272por
dc.subjectDNA barcodingpor
dc.subjectEvolutionarily distinct and globally endangered scorepor
dc.subjectFishpor
dc.subjectFreshwater diversitypor
dc.subjectMediterranean Biodiversity Hotspotpor
dc.subjectMolecular identificationpor
dc.titleSpatial heterogeneity in the Mediterranean Biodiversity Hotspot affects barcoding accuracy of its freshwater fishespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceHobokenpor
oaire.citation.endPage1221por
oaire.citation.startPage1210por
oaire.citation.titleMolecular Ecology Resourcespor
oaire.citation.volume14por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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