Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.12/1791
Título: A rapid and inexpensive molecular technique to discriminate the north-eastern Atlantic and Mediterranean Atherina species and its potential applications in ecology and larval identification
Autor: Almada, Vítor Carvalho
Francisco, Sara Martins
Rosa, Inês
Domingues, Vera dos Santos
Cabral, Henrique
Almada, Frederico
Henriques, Miguel
Robalo, Joana Isabel
Palavras-chave: Atherina
RFLP
mtDNA
Data: 2012
Editora: Springer
Citação: Biologia, 67 (5), 988-991
Resumo: This note describes a rapid and inexpensive Restriction Fragment Length Polymorphism technique to discriminate all species of Atherina (Pisces: Atherinidae) of the north-eastern Atlantic and the Mediterranean. This technique is based on digestion of a fragment of the 12S ribosomal RNA (12SrRNA) gene region of mitochondrial DNA with restriction enzymes that recognize species-specific nucleotide sites. The three currently recognized species in the area, as well as two additional forms awaiting formal description, can be discriminated using a set of four endonucleases. We argue that this simple and fast technique may be of great help in the identification of young stages and in ecological surveys.
Peer review: yes
URI: http://hdl.handle.net/10400.12/1791
ISSN: 0006-3088
Aparece nas colecções:BMAR - Artigos em revistas internacionais

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