Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.12/1465
Título: Extensive intraspecific polymorphism detected by SSCP at the nuclear C-mos gene in the endemic Iberian lizard Lacerta Schreiberi
Autor: Godinho, Raquel
Domingues, Vera dos Santos
Crespo, Eduardo G.
Ferrand, Nuno
Palavras-chave: Intraspecific polymorphism
Lacerta schreiberi
Nuclear gene
Data: 2006
Editora: Blackwell Publishing
Citação: Molecular Ecology, 15, 731-738
Resumo: C-mos is a highly conserved intronless gene that has proved useful in the analysis of ancient phylogenetic relationships within vertebrates. We selected the Iberian endemic Schreiber’s green lizard (Lacerta schreiberi) that persisted in allopatric refugia since the late Pliocene to investigate the utility of the Cmos nuclear gene for intraspecific phylogeographic studies. Our combination of DNA sequencing with the high resolving power of single-strand conformational polymorphism (SSCP) effectively discriminated four common alleles showing strong population structuring (FST = 0.46). In addition, reconstruction of allele phylogenetic relationships further improved our understanding of Cmos spatial patterns of variation and allowed a comparison with previously described mitochondrial DNA data. Finally, limited sequencing of an extended Cmos fragment in six additional Lacerta species showed extensive polymorphism, to our knowledge representing a rare example of variation in a highly conserved nuclear gene.
Peer review: yes
URI: http://hdl.handle.net/10400.12/1465
ISSN: 0962-1083
Aparece nas colecções:UIE-E - Artigos em revistas internacionais

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